hCEACAM5/4T1 hCEACAM5/4T1 hCEACAM5/4T1
I. Background |
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CEACAM 家族由膜結合糖蛋白和分泌性糖蛋白組成。膜結合糖蛋白通過糖磷脂酰肌醇 (GPI)或跨膜結構域錨定在細胞表面。具有 GPI 錨定的成員主要包括 CEACAM5、 CEACAM6、CEACAM7 和 CEACAM8。CEACAM 5 廣泛表達于內皮細胞、上皮細胞、免 疫細胞等多種細胞類型表面。CEACAM 5 在正常組織內主要作為細胞粘附分子,介導同質 和異質性黏附。CEACAM5 編碼的蛋白質可以調節細胞分化、凋亡和細胞極性,從而促進 腫瘤的發展。因此,它也被用作胃腸道癌癥的臨床生物標志物。在癌細胞中,CEACAM5 主要在上皮腫瘤細胞表面高表達,包括結直腸癌、胃癌、胰腺癌、膽囊癌、肺腺癌、小細 胞肺癌、乳腺癌、膀胱癌、卵巢癌等。 |
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II. Introduction |
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Host Cell: | 4T1 |
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Expressed gene: | hCEACAM5 |
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Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
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Freeze Medium: | 90% FBS+10% DMSO |
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Culture Medium: | RPMI-1640+10%FBS+2 μg/ml puromycin |
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Mycoplasma Testing: | Negative |
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Storage: | Liquid nitrogen |
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III. Description of Host Cell Line |
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Organism: | Mouse |
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Tissue: | Mammary gland |
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Morphology: | Epithelial |
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Growth Properties: | Adherent |
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IV. Representative Data |
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Figure 1. Recombinant hCEACAM5/4T1 stably expressing hCEACAM5. |
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