hMSLN/MC-38 hMSLN/MC-38 hMSLN/MC-38
I. Background | | |
間皮素(Mesothelin)又被稱為 MSLN,它是由 MSLN 基因編碼的一種細胞表面糖蛋 白。MSLN 基因編碼一種前體蛋白,經蛋白水解處理生成兩種蛋白產物,主要位于正常間 皮細胞表面,兩種蛋白產物為巨核細胞增強因子(megakaryocyte potentiating,MPF)和間 皮素(Mesothelin,MSLN)。間皮素(MSLN)可黏附于細胞膜上,主要表達于胸腹膜與 臟器的鏈接面上,黏附于間皮細胞表面,在其余正常組織重表達量低。MSLN 在許多實體 瘤中呈現高表達,最常見于間皮瘤、上皮性卵巢癌和胰腺癌,但也見于肺和子宮惡性腫瘤 以及膽管癌。間皮素在卵巢癌和間皮瘤中的表達最高,較高的表達與晚期腫瘤分期和較高的轉移率相關。 | | |
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II. Introduction | | |
Host Cell: | MC-38 | | |
Expressed gene: | hMSLN | | |
Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) | | |
Freeze Medium: | 90% FBS+10% DMSO | | |
Culture Medium: | DMEM+10%FBS+2 μg/ml puromycin | | |
Mycoplasma Testing: | Negative | | |
Storage: | Liquid nitrogen | | |
III. Description of Host Cell Line | | |
Organism: | Mus musculus, mouse | | |
Tissue: | Colon | | |
Morphology: | Epitheloid cell | | |
Growth Properties: | Adherent | | |
IV. Representative Data | | |

Figure 1. Recombinant hMSLN/MC38 stably expressing MSLN. | |
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